Interaction of cryptogein with its binding sites in tobacco plasma membrane studied using the piezoelectric biosensor.
Identifieur interne : 001A82 ( Main/Exploration ); précédent : 001A81; suivant : 001A83Interaction of cryptogein with its binding sites in tobacco plasma membrane studied using the piezoelectric biosensor.
Auteurs : Zuzana Svozilová [République tchèque] ; Tomás Kasparovsk ; Petr Skládal ; Jan LochmanSource :
- Analytical biochemistry [ 1096-0309 ] ; 2009.
Descripteurs français
- KwdFr :
- Liaison aux protéines (MeSH), Membrane cellulaire (métabolisme), Phytophthora (métabolisme), Protéines d'algue (analyse), Protéines d'algue (métabolisme), Protéines fongiques (MeSH), Protéines membranaires (analyse), Protéines membranaires (métabolisme), Protéines végétales (métabolisme), Sites de fixation (MeSH), Tabac (métabolisme), Techniques de biocapteur (méthodes).
- MESH :
- analyse : Protéines d'algue, Protéines membranaires.
- métabolisme : Membrane cellulaire, Phytophthora, Protéines d'algue, Protéines membranaires, Protéines végétales, Tabac.
- méthodes : Techniques de biocapteur.
- Liaison aux protéines, Protéines fongiques, Sites de fixation.
English descriptors
- KwdEn :
- Algal Proteins (analysis), Algal Proteins (metabolism), Binding Sites (MeSH), Biosensing Techniques (methods), Cell Membrane (metabolism), Fungal Proteins (MeSH), Membrane Proteins (analysis), Membrane Proteins (metabolism), Phytophthora (metabolism), Plant Proteins (metabolism), Protein Binding (MeSH), Tobacco (metabolism).
- MESH :
- chemical , analysis : Algal Proteins, Membrane Proteins.
- chemical , metabolism : Algal Proteins, Membrane Proteins, Plant Proteins.
- metabolism : Cell Membrane, Phytophthora, Tobacco.
- methods : Biosensing Techniques.
- Binding Sites, Fungal Proteins, Protein Binding.
Abstract
Elicitins are low-molecular-weight proteins representing the elicitor family secreted by many species of the oomycete Phytophthora. Elicitins induce a hypersensitive reaction in tobacco, a process that is triggered by binding of elicitin to the high-affinity site on the plasma membrane. Specific interaction of cryptogein with the binding sites on tobacco plasma membranes was studied using the piezoelectric biosensor in real time in a flow-through mode. Cryptogeins (wild-type and mutant forms) were covalently immobilized on the sensing surface, and membrane vesicles containing receptors were in solution. Kinetic characterization of the interaction provided values of kinetic rate association (k(a))=5.74 . 10(6)M(1)s(-1) and kinetic rate dissociation (k(d))=6.8710(-4)s(-1) constants, respectively. The kinetic equilibrium dissociation constant was calculated as K(D)=12.0 nM. The piezoelectric biosensor appeared to be a convenient tool for studying interactions of receptors embedded in membrane vesicles.
DOI: 10.1016/j.ab.2009.04.012
PubMed: 19374882
Affiliations:
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uniqKey="Skladal P" first="Petr" last="Skládal">Petr Skládal</name></author><author><name sortKey="Lochman, Jan" sort="Lochman, Jan" uniqKey="Lochman J" first="Jan" last="Lochman">Jan Lochman</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2009">2009</date><idno type="RBID">pubmed:19374882</idno><idno type="pmid">19374882</idno><idno type="doi">10.1016/j.ab.2009.04.012</idno><idno type="wicri:Area/Main/Corpus">001B12</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">001B12</idno><idno type="wicri:Area/Main/Curation">001B12</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">001B12</idno><idno type="wicri:Area/Main/Exploration">001B12</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Interaction of cryptogein with its binding sites in tobacco plasma membrane studied using the piezoelectric biosensor.</title><author><name sortKey="Svozilova, 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(MeSH)</term><term>Protéines membranaires (analyse)</term><term>Protéines membranaires (métabolisme)</term><term>Protéines végétales (métabolisme)</term><term>Sites de fixation (MeSH)</term><term>Tabac (métabolisme)</term><term>Techniques de biocapteur (méthodes)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Algal Proteins</term><term>Membrane Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Algal Proteins</term><term>Membrane Proteins</term><term>Plant Proteins</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Protéines d'algue</term><term>Protéines membranaires</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Cell Membrane</term><term>Phytophthora</term><term>Tobacco</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Biosensing Techniques</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Membrane cellulaire</term><term>Phytophthora</term><term>Protéines d'algue</term><term>Protéines membranaires</term><term>Protéines végétales</term><term>Tabac</term></keywords><keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>Techniques de biocapteur</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Binding Sites</term><term>Fungal Proteins</term><term>Protein Binding</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Liaison aux protéines</term><term>Protéines fongiques</term><term>Sites de fixation</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Elicitins are low-molecular-weight proteins representing the elicitor family secreted by many species of the oomycete Phytophthora. Elicitins induce a hypersensitive reaction in tobacco, a process that is triggered by binding of elicitin to the high-affinity site on the plasma membrane. Specific interaction of cryptogein with the binding sites on tobacco plasma membranes was studied using the piezoelectric biosensor in real time in a flow-through mode. Cryptogeins (wild-type and mutant forms) were covalently immobilized on the sensing surface, and membrane vesicles containing receptors were in solution. Kinetic characterization of the interaction provided values of kinetic rate association (k(a))=5.74 . 10(6)M(1)s(-1) and kinetic rate dissociation (k(d))=6.8710(-4)s(-1) constants, respectively. The kinetic equilibrium dissociation constant was calculated as K(D)=12.0 nM. The piezoelectric biosensor appeared to be a convenient tool for studying interactions of receptors embedded in membrane vesicles.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">19374882</PMID><DateCompleted><Year>2009</Year><Month>08</Month><Day>04</Day></DateCompleted><DateRevised><Year>2012</Year><Month>11</Month><Day>15</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1096-0309</ISSN><JournalIssue CitedMedium="Internet"><Volume>390</Volume><Issue>2</Issue><PubDate><Year>2009</Year><Month>Jul</Month><Day>15</Day></PubDate></JournalIssue><Title>Analytical biochemistry</Title><ISOAbbreviation>Anal Biochem</ISOAbbreviation></Journal><ArticleTitle>Interaction of cryptogein with its binding sites in tobacco plasma membrane studied using the piezoelectric biosensor.</ArticleTitle><Pagination><MedlinePgn>115-20</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.ab.2009.04.012</ELocationID><Abstract><AbstractText>Elicitins are low-molecular-weight proteins representing the elicitor family secreted by many species of the oomycete Phytophthora. Elicitins induce a hypersensitive reaction in tobacco, a process that is triggered by binding of elicitin to the high-affinity site on the plasma membrane. Specific interaction of cryptogein with the binding sites on tobacco plasma membranes was studied using the piezoelectric biosensor in real time in a flow-through mode. Cryptogeins (wild-type and mutant forms) were covalently immobilized on the sensing surface, and membrane vesicles containing receptors were in solution. Kinetic characterization of the interaction provided values of kinetic rate association (k(a))=5.74 . 10(6)M(1)s(-1) and kinetic rate dissociation (k(d))=6.8710(-4)s(-1) constants, respectively. The kinetic equilibrium dissociation constant was calculated as K(D)=12.0 nM. 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last="Kasparovsk">Tomás Kasparovsk</name><name sortKey="Lochman, Jan" sort="Lochman, Jan" uniqKey="Lochman J" first="Jan" last="Lochman">Jan Lochman</name><name sortKey="Skladal, Petr" sort="Skladal, Petr" uniqKey="Skladal P" first="Petr" last="Skládal">Petr Skládal</name></noCountry><country name="République tchèque"><region name="Moravie"><name sortKey="Svozilova, Zuzana" sort="Svozilova, Zuzana" uniqKey="Svozilova Z" first="Zuzana" last="Svozilová">Zuzana Svozilová</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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